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Impact of DMPEI on Biofilm Adhesion on Latex Urinary Catheter

[ Vol. 15 , Issue. 1 ]

Author(s):

Vinícius S. Tarabal, Flávia G. Silva, Ruben D. Sinisterra, Daniel Gonçalves, Jose Silva, Jose M. Granjeiro, Marcelo Speziali and Paulo A. Granjeiro*   Pages 51 - 66 ( 16 )

Abstract:


Background: Microorganisms can migrate from the external environment to the patient’s organism through the insertion of catheters. Despite being indispensable medical device, the catheter surface can be colonized by microorganisms and become a starting point for biofilm formation. Therefore, new technologies are being developed in order to modify surfaces to prevent the adhesion and survival of microorganisms. Patents with the use of DMPEI have been filed.

Objective: In the present work, we coated latex catheter surfaces with 2 mg mL-1 DMPEI in different solvents, evaluated the wettability of the surface and the anti- biofilm activity of the coated catheter against Escherichia coli, Staphylococcus aureus, and Candida albicans.

Methods: We coated the inner and outer catheter surfaces with 2 mg mL-1 of DMPEI solubilized in butanol, dimethylformamide, and cyclohexanone and the surfaces were analyzed visually. Contact angle measurement allowed the analysis of the wettability of the surfaces. The CFU mL-1 count evaluated E. coli, S. aureus, and C. albicans adhesion onto the control and treated surfaces.

Results: The contact angle decreased from 50.48º to 46.93º on the inner surface and from 55.83º to 50.91º on the outer surface of latex catheters coated with DMPEI. The catheter coated with DMPEI showed anti-biofilm activity of 83%, 88%, and 93% on the inner surface and 100%, 92%, and 86% on the outer surface for E. coli, S. aureus, and C. albicans, respectively.

Conclusion: Latex catheter coated with DMPEI efficiently impaired the biofilm formation both on the outer and inner surfaces, showing a potential antimicrobial activity along with a high anti-biofilm activity for medical devices.

Keywords:

CAUTI, UTI, DMPEI, biofilm, urinary catheter, latex.

Affiliation:

Campus Centro-Oeste, Federal University of São João del-Rei, Divinópolis, Minas Gerais, Chemistry Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Chemistry Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Campus Centro-Oeste, Federal University of São João del-Rei, Divinópolis, Minas Gerais, Campus Centro-Oeste, Federal University of São João del-Rei, Divinópolis, Minas Gerais, National Institute of Metrology, Quality and Technology, Duque de Caxias, Rio de Janeiro, Chemistry Department, Federal University of Ouro Preto, Minas Gerais, Campus Centro-Oeste, Federal University of São João del-Rei, Divinópolis, Minas Gerais

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