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Fibrinolytic Enzymes From Extremophilic Microorganisms in the Development of New Thrombolytic Therapies: Technological Prospecting

Author(s):

James Romero Soares Bispo, Igor Gomes de Oliveira Lima, Maurício Bernardo da Silva, Alexya de Oliveira Feitosa, Ana Caroline Melo dos Santos, Magna Suzana Alexandre Moreira, Michel Rodrigo Zambrano Passarini, Paulo Eduardo Aguiar Saraiva Câmara, Luiz Henrique Rosa, Valéria Maia Oliveira, Aline Cavalcanti de Queiroz and Alysson Wagner Fernandes Duarte*   Pages 1 - 15 ( 15 )

Abstract:


Background: Extremophilic microorganisms from a wide variety of extreme natural environments have been researched, and many biotechnological applications have been carried out, due to their capacity to produce biomolecules resistant to extreme conditions, such as fibrinolytic proteases. The search for new fibrinolytic enzymes is important in the development of new therapies against cardiovascular diseases.

Objective: This article aimed to evaluate the patents filed about protease with fibrinolytic activity produced by extremophilic microorganisms whose use is aimed at the development of new drugs for the treatment of cardiovascular diseases.

Methods: The prospecting was carried out using data on deposits and patent concessions made available on the technological bases: European Patent Office (EPO), United States Patent and Trademark Office (USPTO), World Intellectual Property Organization (WIPO), Instituto Nacional de Propriedade Industrial – Brazil (INPI), The LENS and Patent Inspiration. The International Patent Classification and subclasses and groups for each document were also evaluated.

Results: Although 382 patents were selected using terms related to extreme environments, such as “thermophile” and “acidophiles”, few were related to clinical use and were mainly performed using Bacillus subtilis and Streptomyces megasporus strains. A highlight of nattokinase was produced by Bacillus subtilis GDN and actinokinase by Streptomyces megasporus SD5.

Conclusion: The low number of patents on enzymes with this profile (extreme environments) revealed a little-- explored field, promising in the development of new microbial thrombolytic drugs, such as fibrinolytic enzymes with less adverse effects.

Keywords:

Cardiovascular disease, fibrinolytic activity, innovation, pharmaceutical patents, thrombolytic drugs, technological bases.

Affiliation:

Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciências Médicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciências Médicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciências Médicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciências Médicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciências Médicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Laboratório de Farmacologia e Imunologia, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, 57072-900, Maceió, AL, Universidade Federal da Integração Latino-Americana, 85870-650, Foz Do Iguaçu, PR, Departamento de Botânica, Campus Darcy Ribeiro, Universidade de Brasília, Asa Norte, s/n, 70910-900, Brasília, DF, Departamento de Microbiologia, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas. Universidade Estadual de Campinas, UNICAMP, 13148-218, Paulínia, SP, Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciências Médicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciências Médicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL



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