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Designing an Outer Membrane Protein (Omp-W) Based Vaccine for Immunization against Vibrio and Salmonella: An in silico Approach

[ Vol. 14 , Issue. 4 ]

Author(s):

Sadra S. Tehrani, Abolfazl Jahangiri, Mortaza Taheri-Anganeh, Hossein Maghsoudi, Saeed Khalili, Saeed E. Fana, Mahmood Maniati and Jafar Amani*   Pages 312 - 324 ( 13 )

Abstract:


Background: Cholera triggered by Vibrio cholerae remains the main reason for morbidity and mortality all over the world. In addition, salmonellosis is regarded as an infectious disease that makes it essential for the identification and detection of Salmonella. With a beta-barrel structure consisting of eight non-parallel beta strands, OmpW family is widely distributed among gram-negative bacteria. Moreover, OmpW isolated from S. typhimurium and Vibrio cholerae can be used in vaccine design.

Methods: Topology prediction was determined. T-cell and B-cell epitopes were selected from exposed areas, and sequence conservancy was evaluated. The remaining loops and inaccessible residues were removed to prepare OmpW-1. High antigenicity peptides were detected to replace inappropriate residues to obtain OmpW-2. Physicochemical properties were assessed, and antigenicity, hydrophobicity, flexibility, and accessibility were compared to the native Omp-W structure. Low score areas were removed from the designed structure for preparing the OmpW-3. To construct OmpW-4, TTFrC was used as T-CD4+ cell-stimulating factor and CTB as adjuvant to the end of the C-terminal of this sequence, which can increase the antigenicity and sequence density. The sequences were re-analyzed to delete the unfavorable residues. Besides, the solubility of the mature OmpW and the designed structure were predicted while overexpressed in E. coli.

Results: The designed vaccine is a stable protein that has immune cells recognizing epitopes and is considered as an antigen. The construct can be overexpressed in an E. coli.

Conclusion: The multi-epitope vaccine is a suitable stimulator for the immune system and would be a candidate for experimental research. Recent patents describe numerous inventions related to the clinical facets of vaccine peptide against human infectious disease.

Keywords:

Bioinformatics, immune response, O-mpW, salmonella infections, vaccination, vibrio cholera.

Affiliation:

Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Cellular and Molecular Research Center, Research Institute on Cellular and Molecular Medicine, Urmia University of Medical Sciences, Urmia, Student Research Committee, Babol University of Medical Sciences, Babol, Department of Biology Sciences, Shahid Rajaee Teacher Training University, Tehran, Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, English Department, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran

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